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Document And Entity Information - shares	3 Months Ended
Document And Entity Information - shares	Mar. 31, 2016	May 11, 2016
Document Information [Line Items]
Document Type	10-Q
Amendment Flag	false
Document Period End Date	Mar. 31, 2016
Document Fiscal Year Focus	2016
Document Fiscal Period Focus	Q1
Entity Registrant Name	OPGEN INC
Entity Central Index Key	0001293818
Current Fiscal Year End Date	--12-31
Entity Filer Category	Smaller Reporting Company
Trading Symbol	OPGN
Entity Common Stock, Shares Outstanding		12,581,790

Condensed Consolidated Balance Sheets - USD ($)	Mar. 31, 2016	Dec. 31, 2015
Current assets
Cash and cash equivalents	$ 3,967,957	$ 7,814,220
Accounts receivable, net	641,575	678,646
Inventory, net	924,345	826,012
Prepaid expenses and other current assets	456,008	566,239
Total current assets	5,989,885	9,885,117
Property and equipment, net	996,564	1,074,710
Goodwill	600,814	637,528
Intangible assets, net	1,821,860	1,888,814
Other noncurrent assets	270,503	270,327
Total assets	9,679,626	13,756,496
Current liabilities
Accounts payable	2,205,538	2,285,792
Accrued compensation and benefits	1,260,839	1,081,270
Accrued liabilities	930,941	920,286
Deferred revenue	51,794	50,925
Current maturities of long-term capital lease obligation	242,459	251,800
Total current liabilities	4,691,571	4,590,073
Deferred rent	461,552	352,985
Long-term capital lease obligation and other noncurrent liabilities	277,190	328,642
Notes payable	994,792	993,750
Total liabilities	6,425,105	6,265,450
Stockholders' equity
Common stock, $.01 par value; 200,000,000 shares authorized; 12,576,306 and 12,547,644 shares issued and outstanding at March 31, 2016 and December 31, 2015, respectively	125,763	125,477
Preferred stock, $0.01 par value; 10,000,000 shares authorized; none issued at March 31, 2016 and December 31, 2015 (Note 8)	0	0
Additional paid-in capital	121,761,475	121,490,994
Accumulated other comprehensive loss	(2,171)	(1,059)
Accumulated deficit	(118,630,546)	(114,124,366)
Total stockholders’ equity	3,254,521	7,491,046
Total liabilities and stockholders’ equity	$ 9,679,626	$ 13,756,496

Condensed Consolidated Balance Sheets (Parenthetical) - $ / shares	Mar. 31, 2016	Dec. 31, 2015
Preferred stock, par value (in dollars per share)	$ 0.01	$ 0.01
Preferred stock, shares authorized	10,000,000	10,000,000
Preferred stock, shares issued	0	0
Common stock, par value (in dollars per share)	$ 0.01	$ 0.01
Common stock, shares authorized	200,000,000	200,000,000
Common stock, shares issued	12,576,306	12,547,644
Common stock, shares outstanding	12,576,306	12,547,644

Condensed Consolidated Statements of Operations and Comprehensive Loss - USD ($)	3 Months Ended
	Mar. 31, 2016	Mar. 31, 2015
Revenue
Product sales	$ 947,219	$ 184,179
Laboratory services	129,420	35,241
Collaboration revenue	0	252,780
Total revenue	1,076,639	472,200
Operating expenses
Cost of products sold	345,967	115,389
Cost of services	315,709	95,430
Research and development	1,953,429	1,108,602
General and administrative	1,538,046	659,392
Sales and marketing	1,399,435	1,024,029
Total operating expenses	5,552,586	3,002,842
Operating loss	(4,475,947)	(2,530,642)
Other income (expense)
Interest and other income	173	35
Interest expense	(41,734)	(96,397)
Foreign currency transaction gains	11,328	0
Change in fair value of derivative financial instruments	0	31,831
Total other income (expense)	(30,233)	(64,531)
Net loss	(4,506,180)	(2,595,173)
Preferred stock dividends	0	(171,741)
Net loss available to common stockholders	$ (4,506,180)	$ (2,766,914)
Net loss per common share - basic and diluted (in dollars per share)	$ (0.36)	$ (5.61)
Weighted average shares outstanding - basic and diluted (in shares)	12,568,941	493,463
Net loss	$ (4,506,180)	$ (2,595,173)
Other comprehensive loss - foreign currency translation	(1,112)	0
Comprehensive loss	$ (4,507,292)	$ (2,595,173)

Condensed Consolidated Statements of Cash Flows - USD ($)	3 Months Ended
	Mar. 31, 2016	Mar. 31, 2015
Cash flows from operating activities
Net loss	$ (4,506,180)	$ (2,595,173)
Adjustments to reconcile net loss to net cash used in operating activities:
Depreciation and amortization	168,440	106,133
Loss on disposal of property and equipment	6,068	0
Noncash interest expense	1,042	17,123
Share-based compensation	261,497	590,603
Inventory obsolescence	15,034	3,604
Change in fair value of derivative financial instruments	0	(31,831)
Changes in operating assets and liabilities, net of effects of acquisition:
Accounts receivable	39,411	411,999
Inventory	(112,883)	45,331
All other assets	199,182	(25,854)
Accounts payable	(80,276)	(319,483)
Accrued compensation and other liabilities	211,979	145,016
Deferred revenue	869	(64,619)
Net cash used in operating activities	(3,795,817)	(1,717,151)
Cash flows from investing activities
Purchases of property and equipment (net of proceeds on disposals)	(1,644)	(10,034)
Net cash used in investing activities	(1,644)	(10,034)
Cash flows from financing activities
Proceeds from issuance of convertible notes and warrants, net of issuance costs	0	1,388,913
Proceeds from issuance of promissory notes, net of issuance costs	0	500,000
Proceeds from exercise of stock options and warrants	9,271	55
Proceeds from initial public offering, net of issuance costs paid in 2015	0	(252,715)
Payments on debt	0	(1,250)
Payments on capital lease obligations	(63,327)	(25,640)
Net cash provided by (used in) financing activities	(54,056)	1,609,363
Effects of exchange rates on cash	5,254	0
Net decrease in cash and cash equivalents	(3,846,263)	(117,822)
Cash and cash equivalents at beginning of period	7,814,220	749,517
Cash and cash equivalents at end of period	3,967,957	631,695
Supplemental disclosure of cash flow information
Cash paid during the period for interest	13,021	6,865
Supplemental disclosure of noncash investing and financing activities:
Acquisition of equipment purchased through capital leases	0	76,745
Property and equipment purchased on credit	27,767	0
Deferred and accrued financing costs	52,210	218,656
Convertible Debt [Member]
Supplemental disclosure of noncash investing and financing activities:
Exchange of demand note for convertible debt	$ 0	$ 300,000

Organization

3 Months Ended

Mar. 31, 2016

Organization, Consolidation and Presentation of Financial Statements [Abstract]

Organization

Note 1 - Organization

OpGen, Inc. (“OpGen” or the “Company”) was incorporated in Delaware in 2001. On July 14, 2015, OpGen completed the strategic acquisition (the “Merger”) of AdvanDx, Inc. and its wholly owned subsidiary AdvanDx A/S (collectively, “AdvanDx”) (see Note 4). Pursuant to the terms of a merger agreement, Velox Acquisition Corp., OpGen’s wholly owned subsidiary formed for the express purpose of effecting the Merger, merged with and into AdvanDx, Inc. with AdvanDx, Inc. surviving as OpGen’s wholly owned subsidiary. OpGen, AdvanDx, Inc. and AdvanDx A/S are collectively referred to hereinafter as the “Company.” The Company’s headquarters are in Gaithersburg, Maryland, and its principal operations are in Gaithersburg, Maryland and Woburn, Massachusetts. The Company also has operations in Copenhagen, Denmark. The Company operates in one business segment.

OpGen is a precision medicine company using molecular diagnostics and informatics to combat infectious disease. OpGen is developing molecular information solutions to combat infectious disease in global healthcare settings, helping to guide clinicians with more rapid information about life threatening infections, improve patient outcomes, and decrease the spread of infections caused by multidrug-resistant microorganisms. The Company’s proprietary DNA tests and bioinformatics address the rising threat of antibiotic resistance by helping physicians and healthcare providers optimize patient care decisions and protect the hospital biome through customized screening and surveillance solutions. The Company’s molecular information solution combines Acuitas® DNA tests, Acuitas Lighthouse™ bioinformatics and CLIA lab services for MDRO genetic identification, antibiotic resistance gene information and surveillance, and a proprietary data warehouse including genomic data matched with antibiotic susceptibility information for microbes and patient information from healthcare providers. The Company is working to deliver its molecular information products and services to a global network of customers and partners. The Acuitas DNA tests provide rapid microbial ID, and antibiotic resistance gene information. These include the QuickFISH® family of FDA-cleared and CE-marked diagnostic products used to rapidly detect pathogens in positive blood cultures, the MDRO Gene Test to detect, type, track, and trend antibiotic resistant organisms in real-time and a rapid antibiotic resistance test in development.

The Company’s operations are subject to certain risks and uncertainties. The risks include rapid technology changes, the need to manage growth, the need to retain key personnel, the need to protect intellectual property and the need to raise additional capital financing on terms acceptable to the Company. The Company’s success depends, in part, on its ability to develop and commercialize its proprietary technology as well as raise additional capital.

Liquidity and management's plans

3 Months Ended

Mar. 31, 2016

Liquidation Basis Of Accounting Abstract [Abstract]

Liquidity and management’s plans

Note 2 – Liquidity and management’s plans

The accompanying unaudited condensed consolidated financial statements have been prepared on a going-concern basis, which contemplates the realization of assets and satisfaction of liabilities in the normal course of business. Since inception, the Company has incurred, and continues to incur, significant losses from operations. The Company has funded its operations primarily through external investor financing arrangements. The Company raised significant funds in 2015, including:

$0.8 million in short-term notes (in the first quarter of 2015, $0.3 million of demand notes held by an entity controlled by our chief executive officer were settled as partial payment for a 2015 convertible note, and in the second quarter of 2015, $0.2 million of notes from a related party were repaid in cash);

$1.5 million through the issuance of convertible notes;

$12.1 million in net proceeds from its initial public offering (“IPO”) as discussed further below; and

$6.0 million in net proceeds from the issuances of common stock and a senior secured promissory note to Merck Global Health Innovation Fund, LLC (“Merck GHI”).

On May 8, 2015, OpGen completed its IPO pursuant to which it offered and sold 2,850,000 units, each Unit consisting of one share of common stock and a detachable stock purchase warrant to purchase an additional share of common stock, at an initial offering price of $6.00 per unit. Of the total gross proceeds of $17.1 million, approximately $2.1 million was used to satisfy outstanding demand notes by exchanging such notes for 350,000 Units in the IPO. After considering the demand notes, underwriting discounts and commissions and offering expenses, the total net cash proceeds were $12.1 million. On the IPO closing date, the underwriters exercised their over-allotment option to acquire an additional 422,500 stock purchase warrants. In connection with the IPO, all of OpGen’s outstanding Series A Preferred Stock, 2014 convertible notes and 2015 convertible notes were converted into 7,374,852 shares of common stock.

In July 2015, the Company raised $6.0 million by issuing 1,136,364 shares of common stock at $4.40 per share and a $1.0 million senior secured promissory note to Merck GHI. Also in July 2015, the Company entered into a Registration Rights Agreement with Merck GHI and the AdvanDx stockholders who received Merger Consideration in the Merger, which will require the Company to register such shares of Company common stock for resale by such holders in the future. Under the Purchase Agreement, Merck GHI has the right to participate in future securities offerings made by the Company. There is no assurance that Merck GHI will exercise such participation rights in the future.

On May 11, 2016, the Company priced a private placement financing under which, upon closing, it expects to receive $10.4 million upon the issuance of units, each unit consisting of one share of common stock and a warrant to acquire 0.75 of a share of common stock.

Current cash on hand will be sufficient to fund operations into the second quarter of 2016. Once the May 2016 financing is closed, cash on hand , plus the net proceeds from the May 2016 financing, will be sufficient to fund operations into the first quarter of 2017. In the event the Company is unable to successfully raise additional capital in or before the first quarter of 2017, the Company will not have sufficient cash flows and liquidity to finance its business operations as currently contemplated. Accordingly, in such circumstances the Company would be compelled to reduce general and administrative expenses and delay research and development projects, including the purchase of scientific equipment and supplies, until it is able to obtain sufficient financing, or pursue other strategic alternatives which may include licensing and/or partnering arrangements or mergers and acquisitions. The condensed consolidated financial statements do not include any adjustments relating to recoverability and classification of recorded asset amounts or the amounts and classification of liabilities that might be necessary should the Company be unable to continue in existence.

Summary of significant accounting policies

3 Months Ended

Mar. 31, 2016

Accounting Policies [Abstract]

Summary of Significant Accounting Policies

Note 3 - Summary of significant accounting policies

Basis of presentation and consolidation

The accompanying interim condensed consolidated financial statements are unaudited. These unaudited interim condensed consolidated financial statements have been prepared in accordance with the rules and regulations of the U.S. Securities and Exchange Commission (“SEC”) for interim financial information. Accordingly, they do not include all the information and footnotes required by U.S. Generally Accepted Accounting Principles (“GAAP”) for complete financial statements. These unaudited interim condensed consolidated financial statements should be read in conjunction with the audited consolidated financial statements and accompanying notes for the year ended December 31, 2015 previously filed with the SEC. In the opinion of management, the unaudited interim condensed consolidated financial statements reflect all the adjustments (consisting of normal recurring adjustments) necessary to state fairly the Company’s financial position as of March 31, 2016 and the results of operations and cash flows for the three months ended March 31, 2016 and 2015. The interim condensed consolidated results of operations are not necessarily indicative of the results that may occur for the full fiscal year. The December 31, 2015 consolidated balance sheet included herein was derived from the audited consolidated financial statements, but do not include all disclosures including notes required by GAAP for complete financial statements.

The accompanying unaudited interim condensed consolidated financial statements include the accounts of OpGen and its wholly owned and controlled subsidiaries; all intercompany transactions and balances have been eliminated. The Company operates in one business segment. Certain prior period information has been reclassified to conform to the current period presentation.

Foreign Currency

AdvanDx A/S is located in Copenhagen, Denmark and uses the Danish Krone as its functional currency. As a result, all assets and liabilities are translated into U.S. dollars based on exchange rates at the end of the reporting period. Income and expense items are translated at the average exchange rates prevailing during the reporting period. Translation adjustments are reported in accumulated other comprehensive loss, a component of stockholder's equity. Foreign currency translation adjustments are the sole component of accumulated other comprehensive loss at March 31, 2016 and December 31, 2015.

Foreign currency transaction gains and losses, excluding gains and losses on intercompany balances where there is no current intent to settle such amounts in the foreseeable future, are included in the determination of net loss.

Unless otherwise noted, all references to “$” or “dollar” refer to the U.S. dollar.

Use of estimates

In preparing financial statements in conformity with GAAP, management is required to make estimates and assumptions that affect the reported amounts of assets and liabilities and the disclosure of contingent assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the reporting period. In the accompanying consolidated financial statements, estimates are used for, but not limited to, share-based compensation, allowances for doubtful accounts and inventory obsolescence, valuation of derivative financial instruments, beneficial conversion features of convertible debt, deferred tax assets and liabilities and related valuation allowance, and depreciation and amortization and estimated useful lives of long-lived assets, and the recoverability of long-lived assets. Actual results could differ from those estimates.

Fair value of financial instruments

All financial instruments classified as current assets and liabilities are carried at cost, which approximates fair value, because of the short-term maturities of those instruments. The carrying value of the Company’s debt is reflective of fair value based on instruments with similar terms available to the Company.

For additional fair value disclosures, see Note 6.

Cash and cash equivalents

The Company considers all highly liquid instruments with original maturities of three months or less to be cash equivalents. The Company has cash and cash equivalents deposited in financial institutions in which the balances occasionally exceed the federal government agency (FDIC) insured limits of $250,000. The Company has not experienced any losses in such accounts and management believes it is not exposed to any significant credit risk.

At March 31, 2016 and December 31, 2015, the Company has funds totaling $243,380, which are required as collateral for letters of credit benefiting its landlords and for credit card processors. These funds are reflected in other non-current assets on the accompanying condensed consolidated balance sheets.

Accounts receivable

The Company’s accounts receivable result from revenues earned but not collected from customers. Credit is extended based on an evaluation of a customer’s financial condition and, generally, collateral is not required. Accounts receivable are due within 30 to 60 days and are stated at amounts due from customers. The Company evaluates if an allowance is necessary by considering a number of factors, including the length of time accounts receivable are past due, the Company’s previous loss history and the customer’s current ability to pay its obligation. If amounts become uncollectible, they are charged to operations when that determination is made. The allowance for doubtful accounts was $13,352 and $15,596 as of March 31, 2016 and December 31, 2015, respectively.

At March 31, 2016, the Company had no accounts receivable from any individual customer in excess of 10% of total accounts receivable. There was no revenue earned from any individual customer in excess of 10% of total revenue during the three months ended March 31, 2016. For the three months ended March 31, 2015, revenue earned from Hitachi High-Technologies Corporation represented 54% of total revenues; this customer contract was completed as of December 31, 2015.

Inventories

Inventories are valued using the first-in, first-out method and stated at the lower of cost or market and consist of the following:

	March 31, 2016		December 31, 2015

Raw materials and supplies	$	600,044	$	362,526
Work-in process		127,130		150,369
Finished goods		197,171		313,117
Total	$	924,345	$	826,012

Inventory includes the Argus Whole Genome Mapping Systems, reagents and supplies used for Argus consumable kits, reagents and components for QuickFISH and PNA FISH kit products, and reagents and supplies used for the Company’s laboratory services. Inventory reserves for obsolescence and expirations were $614,109 and $599,064 at March 31, 2016 and December 31, 2015, respectively.

Long-lived assets

Property and equipment

Property and equipment is reviewed for impairment whenever events or changes in circumstances indicate that the carrying amount of an asset may not be recoverable. Recoverability of assets to be held and used is measured by a comparison of the carrying amount of an asset to future undiscounted net cash flows expected to be generated by the asset. Recoverability measurement and estimating of undiscounted cash flows is done at the lowest possible level for which we can identify assets. If such assets are considered to be impaired, impairment is recognized as the amount by which the carrying amount of assets exceeds the fair value of the assets. During the three months ended March 31, 2016 and 2015, the Company determined that its property and equipment was not impaired.

Intangible assets and goodwill

Intangible assets and goodwill as of March 31, 2016 were acquired as part of the Merger, and consist of definite-lived intangible assets and goodwill.

Definite-lived intangible assets

Definite-lived intangible assets include trademarks, developed technology and customer relationships, and are amortized over their useful lives of 10, 7 and 7 years, respectively, and consisted of the following as of March 31, 2016:

	Cost		Accumulated Amortization		Net balance at March 31, 2016

Trademarks and tradenames	$	461,000	$	(32,838)	$	428,162
Developed technology		458,000		(46,607)		411,393
Customer relationships		1,094,000		(111,695)		982,305
	$	2,013,000	$	(191,140)	$	1,821,860

Total amortization expense of intangible assets was $66,954 for the three months ended March 31, 2016 (none in the same period of 2015). Amortization of intangible assets is expected to be approximately $268,000 per year for the next five years.

Definite-lived intangible assets are reviewed for impairment whenever events or changes in circumstances indicate that the carrying amount of the asset may not be recoverable. If any indicators were present, the Company would test for recoverability by comparing the carrying amount of the asset to the net undiscounted cash flows expected to be generated from the asset. If those net undiscounted cash flows do not exceed the carrying amount (i.e., the asset is not recoverable), the Company would perform the next step, which is to determine the fair value of the asset and record an impairment loss, if any. During the three months ended March 31, 2016 and 2015, the Company determined that its definite-lived intangible assets were not impaired.

Goodwill

Goodwill represents the excess of the purchase price for AdvanDx over the fair values of the acquired tangible or intangible assets and assumed liabilities. Goodwill is not tax deductible in any relevant jurisdictions. As a result of the Merger and subsequent measurement period adjustments recognized in 2016 and 2015, the Company recognized goodwill of $600,814.

The Company conducts an impairment test of goodwill on an annual basis as of October 1 of each year, and will also conduct tests if events occur or circumstances change that would, more likely than not, reduce the Company’s fair value below its net equity value. During the three months ended March 31, 2016, the Company determined that its goodwill was not impaired.

Redeemable convertible preferred stock

All shares of Series A redeemable convertible preferred stock (“Series A Preferred Stock”) (including those shares issued in connection with the conversion of the 2014 and 2015 convertible debt), were converted into 7,374,852 shares of common stock in connection with the Company’s IPO (see Notes 7 and 8).

Prior to the IPO, the carrying value of the Series A Preferred Stock was increased by the accretion of related discounts, issuance costs and accrued but unpaid dividends so that the carrying amount would equal the redemption amount at the dates the stock becomes redeemable. At March 31, 2015, the Company had 3,999,864 shares of Series A Preferred Stock outstanding. The Series A Preferred Stock was redeemable at the option of the holders of 70% of the outstanding shares of Series A Preferred Stock, subject to certain additional requirements. The Company’s redeemable convertible preferred stock was classified as temporary equity due to redemption provisions outside of the Company’s control.

Revenue recognition

The Company recognizes revenue primarily from sales of its products and services when the following criteria are met: persuasive evidence of an arrangement exists; delivery has occurred; the selling price is fixed or determinable; and collectability is reasonably assured. At times, the Company sells products and services, or performs software development, under multiple-element arrangements with separate units of accounting; in these situations, total consideration is allocated to the identified units of accounting based on their relative selling prices and revenue is then recognized for each unit based on its specific characteristics.

Amounts billed to customers for shipping and handling are included in revenue when the related product or service revenue is recognized. Shipping and handling costs are included in cost of sales.

Revenue from sales of the Argus System

When an Argus System is sold without the Genome Builder software, total arrangement consideration is recognized as revenue when the system is delivered to the customer. Ancillary performance obligations, including installation, limited customer training and limited consumables, are considered inconsequential and are combined with the Argus System as one unit of accounting.

When an Argus System is sold with the Genome Builder software in a multiple-element arrangement, total arrangement consideration is allocated to the Argus System and to the Genome Builder software based on their relative selling prices. Selling prices are determined based on sales of similar systems to similar customers and, where no sales have occurred, on management’s best estimate of the expected selling price relative to similar products. Revenue related to the Argus System is recognized when it is delivered to the customer; revenue for the Genome Builder software is recognized when it is delivered to the customer.

Revenue from sales of QuickFISH, PNA FISH and XpressFISH diagnostic test products

Revenue is recognized upon shipment to the customer. Sales are recorded net of accruals for estimated rebates, discounts and other deductions and returns.

Revenue from sales of Genome Builder Software and consumables (on a stand-alone basis)

Revenue is recognized for Genome Builder Software and for consumables, when sold on a standalone basis, upon delivery to the customer.

Revenue from extended warranty service contracts

The Company recognizes revenue associated with extended warranty service contracts over the service period in proportion to the costs expected to be incurred over that same period.

Revenue from providing laboratory services

The Company recognizes revenue associated with laboratory services contracts when the service has been performed and reports are made available to the customer.

Revenue from funded software development arrangements

The Company’s funded software development arrangements generally consist of multiple elements. Total arrangement consideration is allocated to the identified units of accounting based on their relative selling prices and revenue is then recognized for each unit based on its specific characteristics. When funded software development arrangements include substantive research and development milestones, revenue is recognized for each such milestone when the milestone is achieved and is due and collectible. Milestones are considered substantive if all of the following conditions are met: (1) the milestone is nonrefundable; (2) achievement of the milestone was not reasonably assured at the inception of the arrangement; (3) substantive effort is involved to achieve the milestone; and (4) the amount of the milestone appears reasonable in relation to the effort expended, the other milestones in the arrangement and the related risk associated with achievement of the milestone.

Share-based compensation

Share-based payments are recognized at fair value. The fair value of share-based payments to employees and directors is estimated, on the date of grant, using the Black-Scholes model. The resulting fair value is recognized ratably over the requisite service period, which is generally the vesting period of the option. For all time-vesting awards granted, expense is amortized using the straight-line attribution method. Share-based compensation expense recognized is based on the value of the portion of stock-based awards that is ultimately expected to vest during the period.

Option valuation models, including the Black-Scholes model, require the input of highly subjective assumptions, and changes in the assumptions used can materially affect the grant-date fair value of an award. These assumptions include the risk-free rate of interest, expected dividend yield, expected volatility and the expected life of the award.

Income taxes

Income taxes are accounted for under the asset and liability method. Deferred tax assets and liabilities are recognized for the expected future tax consequences attributable to temporary differences between financial statement carrying amounts of existing assets and liabilities and their respective tax basis. Deferred tax assets and liabilities are measured using enacted tax rates expected to apply to taxable income in the years in which those temporary differences are expected to be recovered or settled. The effect on deferred tax assets and liabilities of a change in tax rates is recognized in income in the period that includes the enactment date. A valuation allowance is established when necessary to reduce deferred income tax assets to the amount expected to be realized.

Tax benefits are initially recognized in the financial statements when it is more likely than not the position will be sustained upon examination by the tax authorities. Such tax positions are initially, and subsequently, measured as the largest amount of tax benefit that is greater than 50% likely of being realized upon ultimate settlement with the tax authority, assuming full knowledge of the position and all relevant facts.

The Company had federal net operating loss (“NOL”) carryforwards of $90,297,000 at December 31, 2015. Despite the NOL carryforwards, which begin to expire in 2022, the Company may have future tax liability due to alternative minimum tax or state tax requirements. Also, use of the NOL carryforwards may be subject to an annual limitation as provided by Section 382 of the Internal Revenue Code of 1986, as amended (the “Code”). To date, the Company has not performed a formal study to determine if any of its remaining NOL and credit attributes might be further limited due to the ownership change rules of Section 382 or Section 383 of the Code. The Company will continue to monitor this matter going forward. There can be no assurance that the NOL carryforwards will ever be fully utilized.

Loss per share

Basic loss per share is computed by dividing net loss available to common stockholders by the weighted average number of shares of common stock outstanding during the period.

For periods of net income, and when the effects are not anti-dilutive, diluted earnings per share is computed by dividing net income available to common stockholders by the weighted-average number of shares outstanding plus the impact of all potential dilutive common shares, consisting primarily of common stock options and stock purchase warrants using the treasury stock method, and convertible preferred stock and convertible debt using the if-converted method.

For periods of net loss, diluted loss per share is calculated similarly to basic loss per share because the impact of all dilutive potential common shares is anti-dilutive. The number of anti-dilutive shares, consisting of (i) common stock options, (ii) stock purchase warrants, and (iii) prior to the IPO, convertible preferred stock and convertible debt, exercisable or exchangeable into common stock which have been excluded from the computation of diluted loss per share, was 5.9 million shares and 8.9 million shares for the three months ended March 31, 2016 and 2015, respectively. The Company’s convertible preferred stock, prior to its conversion, contained non-forfeitable rights to dividends, and therefore was considered to be a participating security; the calculation of basic and diluted income (loss) per share excludes net income (but not net loss) attributable to the convertible preferred stock from the numerator and excludes the impact of those shares from the denominator in periods prior to the IPO.

Recent accounting pronouncements

In May 2014, the Financial Accounting Standards Board (“FASB”) issued guidance for revenue recognition for contracts, superseding the previous revenue recognition requirements, along with most existing industry-specific guidance. The guidance requires an entity to review contracts in five steps: 1) identify the contract, 2) identify performance obligations, 3) determine the transaction price, 4) allocate the transaction price, and 5) recognize revenue. The new standard will result in enhanced disclosures regarding the nature, amount, timing, and uncertainty of revenue arising from contracts with customers. In August 2015, the FASB issued guidance approving a one-year deferral, making the standard effective for reporting periods beginning after December 15, 2017, with early adoption permitted only for reporting periods beginning after December 15, 2016. In March 2016, the FASB issued guidance to clarify the implementation guidance on principal versus agent considerations for reporting revenue gross rather than net, with the same deferred effective date, and in April 2016 the FASB issued guidance to clarify the identification of performance obligations and licensing arrangements. The Company is currently evaluating the impact, if any, that this guidance will have on its financial statements.

In August 2014, the FASB issued guidance requiring management to evaluate on a regular basis whether any conditions or events have arisen that could raise substantial doubt about the entity’s ability to continue as a going concern. The guidance 1) provides a definition for the term “substantial doubt,” 2) requires an evaluation every reporting period, interim periods included, 3) provides principles for considering the mitigating effect of management’s plans to alleviate the substantial doubt, 4) requires certain disclosures if the substantial doubt is alleviated as a result of management’s plans, 5) requires an express statement, as well as other disclosures, if the substantial doubt is not alleviated, and 6) requires an assessment period of one year from the date the financial statements are issued. The standard is effective for the Company’s reporting year beginning January 1, 2017 and early adoption is permitted. The Company is currently evaluating the impact, if any, that this new accounting pronouncement will have on its financial statements.

In April 2015, the FASB issued accounting guidance requiring that debt issuance costs related to a recognized liability be presented on the balance sheet as a direct reduction from the carrying amount of that debt liability, consistent with debt discounts. The recognition and measurement guidance for debt issuance costs are not affected. The standard is effective for reporting periods beginning after December 15, 2015. The Company adopted this guidance effective January 1, 2016 on a retrospective basis, and all periods are presented under this guidance.

In April 2015, the FASB issued guidance as to whether a cloud computing arrangement (e.g., software as a service, platform as a service, infrastructure as a service, and other similar hosting arrangements) includes a software license and, based on that determination, how to account for such arrangements. If a cloud computing arrangement includes a software license, then the customer should account for the software license element of the arrangement consistent with the acquisition of other software licenses. If a cloud computing arrangement does not include a software license, the customer should account for the arrangement as a service contract. The guidance is effective for reporting periods beginning after December 15, 2015, and can be adopted on either a prospective or retrospective basis. The Company adopted this guidance for the year ended December 31, 2016, on a prospective basis. The adoption of this new guidance did not have a material impact on the Company’s financial statements.

In July 2015, the FASB issued accounting guidance for inventory. Under the guidance, an entity should measure inventory within the scope of this guidance at the lower of cost and net realizable value, except when inventory is measured using LIFO or the retail inventory method. Net realizable value is the estimated selling price in the ordinary course of business, less reasonably predictable costs of completion, disposal, and transportation. In addition, the FASB has amended some of the other inventory guidance to more clearly articulate the requirements for the measurement and disclosure of inventory. The standard is effective for reporting periods beginning after December 15, 2016. The amendments in this pronouncement should be applied prospectively, with earlier application permitted. The Company is currently evaluating the impact, if any, that this new accounting pronouncement will have on its financial statements.

In February 2016, the FASB issued guidance for the accounting for leases. The guidance requires lessees to recognize assets and liabilities related to long-term leases on the balance sheet and expands disclosure requirements regarding leasing arrangements. The guidance is effective for reporting periods beginning after December 15, 2018 and early adoption is permitted. The guidance must be adopted on a modified retrospective basis and provides for certain practical expedients. The Company is currently evaluating the impact, if any, that this new accounting pronouncement will have on its financial statements.

In March 2016, the FASB issued guidance to clarify the requirements for assessing whether contingent call or put options that can accelerate the payment of principal on debt instruments are clearly and closely related to their debt hosts. The guidance is effective for reporting periods beginning after December 15, 2016, and early adoption is permitted. Entities are required to apply the guidance to existing debt instruments using a modified retrospective transition method as of beginning of the fiscal year of adoption. The Company is currently evaluating the impact, if any, that this new accounting pronouncement will have on its financial statements.

In March 2016, the FASB issued guidance simplifying the accounting for and financial statement disclosure of stock-based compensation awards. Under the guidance, all excess tax benefits and tax deficiencies related to stock-based compensation awards are to be recognized as income tax expenses or benefits in the income statement and excess tax benefits should be classified along with other income tax cash flows in the operating activities section of the statement of cash flows. Under the guidance, companies can also elect to either estimate the number of awards that are expected to vest or account for forfeitures as they occur. In addition, the guidance amends some of the other stock-based compensation awards guidance to more clearly articulate the requirements and cash flow presentation for withholding shares for tax-withholding purposes. The guidance is effective for reporting periods beginning after December 15, 2016 and early adoption is permitted, though all amendments of the guidance must be adopted in the same period. The adoption of certain amendments of the guidance must be applied prospectively, and adoption of the remaining amendments must be applied either on a modified retrospective basis or retrospectively to all periods presented. The Company is currently evaluating the impact, if any, that this new accounting pronouncement will have on its financial statements.

The Company has evaluated all other issued and unadopted Accounting Standards Updates and believes the adoption of these standards will not have a material impact on its results of operations, financial position, or cash flows.

Business Combination

3 Months Ended

Mar. 31, 2016

Business Combinations [Abstract]

Business Combination

Note 4 – Business Combination

On July 14, 2015, the Company acquired 100% of the capital stock of AdvanDx in the Merger in a taxable transaction. AdvanDx researches, develops and markets advanced in vitro diagnostic kits for the diagnosis and prevention of infectious diseases, and sells its products principally to hospitals and clinical laboratories in the United States and Europe. The Company acquired AdvanDx principally to use AdvanDx’s diagnostic capabilities with respect to MDROs and leverage AdvanDx’s relationships with hospitals and clinical laboratories to accelerate the sales of OpGen’s products and services.

Pursuant to an Agreement and Plan of Merger (the “Merger Agreement”), Velox Acquisition Corp. merged with and into AdvanDx, Inc. with AdvanDx, Inc. surviving as a wholly owned subsidiary of the Company in accordance with the General Corporation Law of the State of Delaware. Under the terms of the Merger Agreement, the merger consideration consisted of an aggregate 681,818 shares of the Company’s common stock with a value of $2.6 million (the “Merger Consideration”), which Merger Consideration was distributed in accordance with the liquidation preferences set forth in the AdvanDx, Inc. Restated Certificate of Incorporation, as amended.

The Company accounted for its acquisition of AdvanDx by recording all tangible and intangible assets acquired and liabilities assumed at their estimated fair values on the acquisition date, with the remaining unallocated purchase price recorded as goodwill. The fair value assigned to identifiable intangible assets acquired was determined using an income approach for trade names and customer relationships, and a cost approach for technology. The fair values are preliminary, are based on the Company’s estimates and may be adjusted from time to time, but no later than July 13, 2016, as better information becomes available. The Company received carryover tax basis in the acquired assets and liabilities and no tax basis in the intangible assets (including goodwill) established on the acquisition date. As a result, the Company recognized deferred tax assets related to foreign taxing jurisdictions of $4.3 million (fully offset by a corresponding valuation allowance) and net deferred tax liabilities of $0.1 million in the U.S. taxing jurisdiction. The net deferred tax liability in the U.S. taxing jurisdiction resulted in an income tax benefit related to a reduction in the Company’s previously established valuation allowance (which reduction is accounted for outside of purchase accounting). The following represents the preliminary allocation of the purchase price (as adjusted for measurement period adjustments):

Total purchase price - fair value of common stock issued	$	2,584,090

Fair value of tangible assets acquired:
Cash	$	1,367,211
Receivables		536,406
Inventory		881,273
Property and equipment		245,479
Other assets		359,587
Fair value of identifable intangible assets acquired:
Customer relationships		1,094,000
Developed technology		458,000
Trademarks and tradenames		461,000
Fair value of goodwill		600,814

Deferred tax liabilities, net		129,095
Fair value of liabilities assumed		3,290,585
	$	2,584,090

The total consideration paid in the acquisition exceeded the estimated fair value of the tangible and identifiable intangible assets acquired and liabilities assumed, resulting in approximately $0.6 million of goodwill. Goodwill, primarily related to expected synergies gained from combining operations, sales growth from future product offerings and customers, together with certain intangible assets that do not qualify for separate recognition, including assembled workforce, is not tax deductible. The Company expensed acquisition-related costs of approximately $0.5 million related to the Merger in 2015.

Adjustments to Goodwill

In the fourth quarter of 2015, the Company adopted new accounting guidance with respect to the accounting for measurement period adjustments resulting from business combinations. Under the new guidance, the Company is required to recognize adjustments to provisional amounts identified during the measurement period in the reporting period in which the adjustments are determined and disclose the portion of the amount recorded in current-period losses by line item that would have been recorded in previous reporting periods if the adjustment had been recognized as of the acquisition date.

During the fourth quarter of 2015, as a result of obtaining new information about facts and circumstances that existed as of the acquisition date, the Company adjusted the provisional estimated fair values of certain acquired assets and liabilities acquired in the Merger, resulting in an increase in goodwill recognized by $345,781. During the first quarter of 2016, the Company identified an additional adjustment to the provisional estimated fair values, as follows:

	As previously reported (1)		Adjustment		Adjusted amount

Accounts payable	$	2,285,792	$	(36,714)	$	2,249,078
Goodwill		637,528		(36,714)		600,814

(1) As reported on Form 10-K for the year ended December 31, 2015

Pro Forma Disclosures (unaudited)

The following unaudited pro forma financial information summarizes the results of operations for the three months ended March 31, 2015 as if the Merger had been completed as of January 1, 2015. Pro forma information primarily reflects adjustments relating to (i) elimination of the interest on AdvanDx’s outstanding debt, and (ii) the amortization of intangibles acquired. The pro forma amounts do not purport to be indicative of the results that would have actually been obtained if the acquisition occurred as of January 1, 2015 or that may be obtained in the future:

	Three Months Ended March 31,
Unaudited pro forma results	2015

Revenues	$	1,429,686
Net loss	$	(3,797,029)
Net loss per share	$	(3.38)

- Definition

The entire disclosure for a business combination (or series of individually immaterial business combinations) completed during the period, including background, timing, and recognized assets and liabilities. The disclosure may include leverage buyout transactions (as applicable).

+ References

Reference 1: http://www.xbrl.org/2003/role/presentationRef
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+ Details

Merck GHI Financing

3 Months Ended

Mar. 31, 2016

Common Stock and Note Purchase Agreement [Abstract]

Merck GHI Financing

Note 5 – Merck GHI Financing

On July 14, 2015, as a condition to the Merger, the Company entered into a Common Stock and Note Purchase Agreement (the “Purchase Agreement”) with Merck GHI, pursuant to which Merck GHI purchased 1,136,364 shares of common stock of the Company at $4.40 per share for gross proceeds of $5.0 million. Pursuant to the Purchase Agreement, the Company also issued to Merck GHI a 8% Senior Secured Promissory Note (the “Note”) in the principal amount of $1.0 million with a two-year maturity date from the date of issuance. The Company’s obligations under the Note are secured by a lien on all of the Company’s assets.

The Company incurred issuance costs of approximately $50,000 related to the financing, of which approximately $8,000 was deferred as debt issuance costs and netted against notes payable in the accompanying condensed consolidated balance sheets as a result of the Company’s adoption of new accounting guidance in 2016, and are being amortized as interest expense over the life of the Note, and $42,000 was charged to additional paid-in capital.

Fair value measurements

3 Months Ended

Mar. 31, 2016

Fair Value Disclosures [Abstract]

Fair value measurements

Note 6 - Fair value measurements

The Company classifies its financial instruments using a three-tier fair value hierarchy, which prioritizes the inputs used in measuring fair value. These tiers include:

⋅

Level 1 - defined as observable inputs such as quoted prices in active markets;

⋅

Level 2 - defined as inputs other than quoted prices in active markets that are either directly or indirectly observable; and

⋅

Level 3 - defined as unobservable inputs in which little or no market data exists, therefore requiring an entity to develop its own assumptions such as expected revenue growth and discount factors applied to cash flow projections.

Financial assets and liabilities measured at fair value on a recurring basis

The Company evaluates financial assets and liabilities subject to fair value measurements on a recurring basis to determine the appropriate level at which to classify them each reporting period. This determination requires the Company to make subjective judgments as to the significance of inputs used in determining fair value and where such inputs lie within the hierarchy.

Prior to its IPO, certain stock purchase warrants contained cash settlement features and, accordingly, the Company considered them to be derivative financial instruments and accounted for them at fair value using level 3 inputs. As a result of the Company’s IPO and elimination of the cash settlement features pursuant to their terms, those stock purchase warrants were reclassified to equity. For periods prior to the IPO, the Company determined the fair value of these derivative liabilities using a hybrid valuation method that consisted of a probability weighted expected return method that values the Company’s equity securities assuming various possible future economic outcomes while using an option pricing method (that treated all equity linked instruments as call options on the Company’s equity value with exercise prices based on the liquidation preference of the Series A Preferred Stock ) to estimate the allocation of value within one or more of the scenarios. Using this hybrid method, unobservable inputs included the Company’s equity value, the exercise price for each option value, expected timing of possible economic outcomes such as initial public offering, risk free interest rates and stock price volatility. The following tables set forth a summary of changes in the fair value of Level 3 liabilities measured at fair value on a recurring basis for the year ended December 31, 2015:

	Balance at		Established		Change in		Reclassified		Balance at
Description	December 31, 2014		in 2015		Fair Value		to Equity		December 31, 2015

Derivative warrant liability	$	-	$	72,333	$	647,342	$	(719,675)	$	-

The Company has no financial assets and liabilities measured at fair value on a recurring basis as of March 31, 2016.

Financial assets and liabilities carried at fair value on a non-recurring basis

The Company does not have any financial assets and liabilities measured at fair value on a non-recurring basis.

Non-financial assets and liabilities carried at fair value on a recurring basis

The Company does not have any non-financial assets and liabilities measured at fair value on a recurring basis.

Non-financial assets and liabilities carried at fair value on a non-recurring basis

The Company measures its long-lived assets, including property and equipment and intangible assets (including goodwill), at fair value on a non-recurring basis when they are deemed to be impaired. No such fair value impairment was recognized in the three months ended March 31, 2016 and 2015.

See Note 4 for a discussion of the fair value of assets acquired and liabilities assumed in the Merger.

- Definition

The entire disclosure for the fair value of financial instruments (as defined), including financial assets and financial liabilities (collectively, as defined), and the measurements of those instruments as well as disclosures related to the fair value of non-financial assets and liabilities. Such disclosures about the financial instruments, assets, and liabilities would include: (1) the fair value of the required items together with their carrying amounts (as appropriate); (2) for items for which it is not practicable to estimate fair value, disclosure would include: (a) information pertinent to estimating fair value (including, carrying amount, effective interest rate, and maturity, and (b) the reasons why it is not practicable to estimate fair value; (3) significant concentrations of credit risk including: (a) information about the activity, region, or economic characteristics identifying a concentration, (b) the maximum amount of loss the entity is exposed to based on the gross fair value of the related item, (c) policy for requiring collateral or other security and information as to accessing such collateral or security, and (d) the nature and brief description of such collateral or security; (4) quantitative information about market risks and how such risks are managed; (5) for items measured on both a recurring and nonrecurring basis information regarding the inputs used to develop the fair value measurement; and (6) for items presented in the financial statement for which fair value measurement is elected: (a) information necessary to understand the reasons for the election, (b) discussion of the effect of fair value changes on earnings, (c) a description of [similar groups] items for which the election is made and the relation thereof to the balance sheet, the aggregate carrying value of items included in the balance sheet that are not eligible for the election; (7) all other required (as defined) and desired information.

+ References + Details

Debt

3 Months Ended

Mar. 31, 2016

Debt Disclosure [Abstract]

Debt

Note 7 - Debt

All the Company’s outstanding demand notes and convertible notes were exchanged for units in the Company’s IPO or otherwise were converted into Series A Preferred Stock and subsequently converted into shares of common stock in connection with the IPO. A short-term 8% promissory note for $150,000 issued in April 2015 was repaid in cash in June 2015. In July 2015, the Company issued a $1.0 million 8% senior secured promissory note to Merck GHI (see Note 5). As of March 31, 2016 and December 31, 2015, the only debt outstanding was the $1.0 million Notes with Merck GHI that are due in July 2017.

In 2009, the Company entered into loan agreements with the Department of Business and Economic Development, a principal department of the State of Maryland, and Montgomery County, Maryland. Under the terms of the agreements, the State of Maryland and Montgomery County loaned the Company $100,000 and $10,000, respectively, to assist in the relocation of the Company’s operations from Wisconsin to Gaithersburg, Maryland. Interest on the loans accrued at 3%. The interest was deferred and the loans were forgivable under certain conditions, including the Company maintaining operations in Gaithersburg, Maryland, and attaining a specified level of staffing at that site on or before December 31, 2012. The Company did not attain the required level of staffing at December 31, 2012, and, as a result, these notes and accrued interest became due in 2013. The Company negotiated a settlement with the State of Maryland under which it paid $75,000 in June 2013 in full satisfaction of the $100,000 loan principal balance and accrued interest of $11,811. The Company also negotiated a settlement with Montgomery County under which accrued interest due under the loan provisions was forgiven and the loan would be paid in equal quarterly installments over the eight quarters ending December 31, 2015. The loan was paid in full during 2015 and no amounts remain outstanding.

Demand notes

In the fourth quarter of 2014 and first quarter of 2015, the Company raised a total of $2.3 million through the issuance of short-term demand notes. In the first quarter of 2015, $0.3 million of demand notes, held by an entity controlled by our chief executive officer, were settled as partial payment for a 2015 convertible note. All outstanding demand notes were tendered as payment for 350,000 units in the Company’s IPO (see Note 8). Prior to settlement, the demand notes bore interest at 8% per annum, had a first priority security interest in the assets of the Company, and a term of approximately four months.

2014 convertible debt

In July, August and September 2014, the Company raised $1.5 million through the issuance of convertible debt. All outstanding 2014 convertible debt was converted into Series A Preferred Stock and then into 1,500,000 shares of common stock in connection with the Company’s IPO (see Note 8). Prior to its conversion, the debt was convertible, at the option of the holders or in certain cases at the Company’s option, into shares of Series A Preferred Stock or other potential equity securities, bore interest at 8% and was due in full on July 11, 2015.

2015 convertible debt

In February and March 2015, the Company raised $1.5 million in capital through the issuance of 8% secured convertible notes with detachable stock purchase warrants. All outstanding 2015 convertible debt was converted into Series A Preferred Stock and then into 1,875,000 shares of common stock in connection with the Company’s IPO (see Note 8). Prior to its conversion, the 2015 convertible notes were prepayable by the Company without penalty at any time following the three-month anniversary of the closing of the IPO (provided that before the six-month anniversary of the closing of an IPO, the 2015 convertible notes could only be prepaid out of newly issued capital subsequent to the IPO), and were puttable by the holder to the Company in the event of a defined default. The 2015 convertible notes were each convertible, at the election of the holder, into (i) shares of Series A Preferred Stock, at a conversion rate of 1.25 shares of Series A Preferred Stock for each $1.00 converted if the conversion occurs prior to closing of an IPO, or (ii) shares of common stock at a conversion rate of one share of common stock for each $1.00 converted if the conversion occurs after the closing of an IPO.

The conversion option embedded in the convertible notes was determined to contain beneficial conversion features, resulting in the bifurcation of those features as an equity instrument (resulting in an additional debt discount) at issuance. After allocation of the gross proceeds to the detachable stock purchase warrants (discussed below) and beneficial conversion feature, the total debt discount recognized was equal to the face value of the 2015 convertible notes. Upon conversion in May 2015, the remaining unamortized beneficial conversion feature of approximately $1.5 million was charged to interest expense in the accompanying consolidated statement of operations. Remaining unamortized deferred financing costs of $71,421 were also charged to interest expense upon conversion.

The 2015 convertible note holders also received detachable stock purchase warrants exercisable for 225,011 shares of common stock at 110% of the IPO price and exercisable only if the IPO occurred, and then exercisable beginning on the six-month anniversary of the closing of the IPO. Prior to the IPO, as a result of net settlement features, the stock purchase warrants were considered derivative liabilities, were initially recorded at fair value (resulting in a debt discount) and were marked-to-market at each balance sheet date through earnings. As a result of the elimination of the net settlement features in the IPO, the stock purchase warrants were marked to fair value of $0.7 million on May 8, 2015 and then reclassified to equity.

Total interest expense on all debt instruments was $31,934 and $96,397 in the three months ended March 31, 2016 and 2015, respectively.

Stockholders' equity

3 Months Ended

Mar. 31, 2016

Stockholders' Equity Note [Abstract]

Stockholders' Deficit

Note 8 - Stockholders’ equity

As of March 31, 2016, the Company has 200,000,000 shares of authorized common shares and 12,576,306 issued and outstanding, and 10,000,000 of authorized preferred shares, none of which were issued or outstanding.

On May 8, 2015, the Company completed its IPO pursuant to which the Company offered and sold 2,850,000 units, each Unit consisting of one share of common stock and a detachable stock purchase warrant to purchase an additional share of common stock, at an initial offering price of $6.00 per unit. Of the total gross proceeds of $17.1 million, approximately $2.1 million was used to satisfy outstanding demand notes by exchanging such notes for 350,000 Units in the IPO. After considering the demand notes, and underwriting discounts, commissions and offering expenses of $2.9 million (which were charged to additional paid-in capital), the total net cash proceeds to the Company was $12.1 million. On the IPO closing date, the underwriters exercised a portion of their over-allotment option to acquire an additional 422,500 stock purchase warrants for cash of $4,225. In connection with the IPO, all of the Company’s outstanding Series A Preferred Stock, 2014 convertible notes and 2015 convertible notes were converted into 7,374,852 shares of common stock.

The stock purchase warrants issued as part of the units (including over-allotment option) are exercisable for 3,272,500 shares of common stock at $6.60 per share beginning six months after the closing of the IPO for five years, expiring on May 8, 2020. Additionally, the Company issued additional warrants to its investment bankers to purchase 185,250 shares of common stock, on the same terms as the warrants issued with the units. The warrants were valued using the Black-Scholes option pricing model and are classified as equity.

In July 2015, the Company issued 1,136,364 shares of common stock to Merck GHI for cash consideration of $5.0 million (see Note 5).

Stock options

In 2002, the Company adopted the 2002 Stock Option and Restricted Stock Plan (the “2002 Plan”), pursuant to which the Company’s Board of Directors could grant either incentive stock options or non-qualified stock options, shares of restricted stock, shares of unrestricted common stock, and other share-based awards to officers and employees. In 2008, the Company adopted the 2008 Stock Option and Restricted Stock Plan (the “2008 Plan”), pursuant to which the Company’s Board of Directors may grant either incentive or non-qualified stock options or shares of restricted stock to directors, key employees, consultants and advisors.

In April 2015, the Company adopted, and the Company’s stockholders approved, the 2015 Equity Incentive Plan (the “2015 Plan”); the 2015 Plan became effective upon the execution and delivery of the underwriting agreement for the Company’s IPO. Following the effectiveness of the 2015 Plan, no further grants will be made under the 2002 Plan or 2008 Plan. The 2015 Plan provides for the granting of incentive stock options within the meaning of Section 422 of the Internal Revenue Code to employees and the granting of non-qualified stock options to employees, non-employee directors and consultants. The 2015 Plan also provides for the grants of restricted stock, restricted stock units, stock appreciation rights, dividend equivalents and stock payments to employees, non-employee directors and consultants.

Under the 2015 Plan, the aggregate number of shares of the common stock authorized for issuance may not exceed (1) 1,355,000 plus (2) the sum of the number of shares subject to outstanding awards under the 2008 Plan as of the 2015 Plan’s effective date, that are subsequently forfeited or terminated for any reason before being exercised or settled, plus (3) the number of shares subject to vesting restrictions under the 2008 Plan as of the 2015 Plan’s effective date that are subsequently forfeited. In addition, the number of shares that have been authorized for issuance under the 2015 Plan will be automatically increased on the first day of each fiscal year beginning on January 1, 2016 and ending on (and including) January 1, 2025, in an amount equal to the lesser of (1) 4% of the outstanding shares of common stock on the last day of the immediately preceding fiscal year, or (2) another lesser amount determined by the Company’s Board of Directors. Shares subject to awards granted under the 2015 Plan that are forfeited or terminated before being exercised or settled, or are not delivered to the participant because such award is settled in cash, will again become available for issuance under the 2015 Plan. However, shares that have actually been issued shall not again become available unless forfeited. As of March 31, 2016, 748,479 shares remain available for issuance under the 2015 Plan, w hich includes 501,907 shares automatically added to the 2015 Plan on January 1, 2016.

For the three months ended March 31, 2016 and 2015, the Company recorded $0.3 million and $0.6 million, respectively, of stock compensation expense. No income tax benefit for stock-based compensation arrangements was recognized in the condensed consolidated statements of operations due to the Company’s net loss position. The allocation of share-based compensation expense by operating expenses is as follows:

	Three months ended March 31,
	2016		2015
Costs of services	$	4,312	$	-
Research and development		62,218		36,456
General and administrative		172,103		83,299
Sales and marketing		22,864		470,848
	$	261,497	$	590,603

During the three months ended March 31, 2016, the Company granted stock options to acquire 30,000 shares of common stock at a weighted average exercise price of $1.53 per share. The 2016 awards had a weighted average grant date fair value per share of $0.73. The Company has total stock options to acquire 2,119,140 shares of common stock outstanding at March 31, 2016.

Restricted stock units

In March 2014, the Company awarded restricted stock units to acquire 130,640 shares of common stock to its Chief Executive Officer (“CEO”). The restricted stock units were compensation for his service as CEO from October 2013 through June 2014 and were subject to forfeiture if he did not continue to perform management services through October 24, 2014. The restricted stock units vested on October 24, 2014 and 130,640 shares of common stock were issued to the CEO. In the fourth quarter of 2015, the Company granted additional restricted stock units to acquire 75,000 shares of common stock, with a weighted average grant date fair value of $1.70 per share, all of which remain outstanding as of March 31, 2016.

Stock purchase warrants

At March 31, 2016, the following warrants to purchase shares of common stock were outstanding:

Issuance	Exercise Price		Expiration	Shares of Common Stock Subject to Warrants
August 2007	$	7.91	August 2017		8,921
March 2008	$	790.54	March 2018		46
November 2009	$	7.91	November 2019		6,674
January 2010	$	7.91	January 2020		6,674
March 2010	$	7.91	March 2020		1,277
November 2011	$	7.91	November 2021		5,213
December 2011	$	7.91	December 2021		664
March 2012	$	109.90	March 2019		4,125
February 2015	$	6.60	February 2025		225,011
May 2015	$	6.60	May 2020		3,457,750
					3,716,355

The warrants listed above were issued in connection with various debt, preferred stock or development contract agreements. The warrants issued in February 2015 were initially classified as a liability since the exercise price was variable. The exercise price became fixed as a result of the Company’s IPO and, as such, the warrant liability was marked to fair value at that time and reclassified to equity (see Note 6).

- Definition

The entire disclosure for shareholders' equity comprised of portions attributable to the parent entity and noncontrolling interest, including other comprehensive income. Includes, but is not limited to, balances of common stock, preferred stock, additional paid-in capital, other capital and retained earnings, accumulated balance for each classification of other comprehensive income and amount of comprehensive income.

+ References

Reference 1: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 235
-SubTopic 10
-Section S99
-Paragraph 1
-Subparagraph (SX 210.4-08.(d),(e))
-URI http://asc.fasb.org/extlink&oid=26873400&loc=d3e23780-122690

Reference 2: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 505
-SubTopic 10
-Section 50
-Paragraph 4
-URI http://asc.fasb.org/extlink&oid=6928386&loc=d3e21484-112644

Reference 3: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 310
-SubTopic 10
-Section S99
-Paragraph 2
-Subparagraph (SAB TOPIC 4.E)
-URI http://asc.fasb.org/extlink&oid=27010918&loc=d3e74512-122707

Reference 4: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 505
-SubTopic 10
-Section 50
-Paragraph 5
-URI http://asc.fasb.org/extlink&oid=6928386&loc=d3e21488-112644

Reference 5: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 210
-SubTopic 10
-Section S99
-Paragraph 1
-Subparagraph (SX 210.5-02.29-31)
-URI http://asc.fasb.org/extlink&oid=6877327&loc=d3e13212-122682

Reference 6: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 505
-SubTopic 10
-Section S99
-Paragraph 4
-Subparagraph (SAB TOPIC 4.C)
-URI http://asc.fasb.org/extlink&oid=27012166&loc=d3e187143-122770

Reference 7: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 505
-SubTopic 10
-Section 50
-Paragraph 6
-URI http://asc.fasb.org/extlink&oid=6928386&loc=d3e21506-112644

Reference 8: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 505
-SubTopic 10
-Section S99
-Paragraph 1
-Subparagraph (SX 210.3-04)
-URI http://asc.fasb.org/extlink&oid=27012166&loc=d3e187085-122770

Reference 9: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 505
-SubTopic 30
-Section 50
-Paragraph 2
-URI http://asc.fasb.org/extlink&oid=6405834&loc=d3e23285-112656

Reference 10: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 505
-SubTopic 10
-Section 50
-Paragraph 3
-URI http://asc.fasb.org/extlink&oid=6928386&loc=d3e21475-112644

Reference 11: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Glossary Preferred Stock
-URI http://asc.fasb.org/extlink&oid=6521494

Reference 12: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 505
-SubTopic 10
-Section 50
-Paragraph 2
-URI http://asc.fasb.org/extlink&oid=6928386&loc=d3e21463-112644

Reference 13: http://www.xbrl.org/2003/role/presentationRef
-Publisher FASB
-Name Accounting Standards Codification
-Topic 505
-SubTopic 10
-Section 50
-Paragraph 11
-URI http://asc.fasb.org/extlink&oid=6928386&loc=d3e21564-112644

Reference 14: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Staff Accounting Bulletin (SAB)
-Number Topic 4
-Section E

Reference 15: http://www.xbrl.org/2003/role/presentationRef
-Publisher SEC
-Name Staff Accounting Bulletin (SAB)
-Number Topic 4
-Section C

+ Details

Commitments and contingencies

3 Months Ended

Mar. 31, 2016

Commitments and Contingencies Disclosure [Abstract]

Commitments and contingencies

Note 9 - Commitments and contingencies

Operating leases

During the second quarter 2015, the Company extended the term of its Gaithersburg, Maryland office lease, effective May 7, 2015, through January 31, 2021, with one additional five-year renewal at the Company’s election. The Company is responsible for all utilities, repairs, insurance, and taxes under this operating lease. Effective July 1, 2015, the Company further modified its lease agreement to add additional leased space to its headquarters. The Company also leases a facility in Woburn, Massachusetts under an operating lease that expires in January 2017, and provides the Company with options to extend the lease beyond the current expiration date. Additionally, the Company leases office space in Denmark; this lease is currently on a month-to-month basis. Rent expense under the Company’s facility operating leases for the three months ended March 31, 2016 and 2015 was $254,044 and $183,154, respectively.

Capital leases

The Company leases computer equipment, office furniture, and equipment under various capital leases. The leases expire at various dates through 2020. The leases require monthly principal and interest payments.

Registration and other shareholder rights

In connection with the Merger and the investment transactions (see Notes 4 and 5), the Company entered into a Registration Rights Agreement with the AdvanDx stockholders receiving Merger Consideration and with Merck GHI, pursuant to which the investors were granted certain demand registration rights and piggyback registration rights in connection with subsequent registered offerings of the Company’s common stock. Merck GHI also received rights to participate on a pro-rata basis in future securities offerings by the Company.

On December 18, 2013, the Company entered into the Third Amended and Restated Investors’ Rights Agreement (the “Investors’ Rights Agreement”) with investors acquiring promissory notes convertible into shares of the Company's Series A Preferred Stock. Following the IPO, the holders of 20% or more of such shares of common stock subject to the Investors’ Rights Agreement have demand registration rights and piggyback registration rights in connection with subsequent registered offerings of the Company’s common stock.

License agreements, research collaborations and development agreements

3 Months Ended

Mar. 31, 2016

License Agreements Research Collaborations And Development Agreements [Abstract]

License Agreements, Research Collaborations and Development Agreements

Note 10 - License agreements, research collaborations and development agreements

The Company is a party to three license agreements to acquire certain patent rights and technologies. Royalties are incurred upon the sale of a product or service which utilizes the licensed technology. Certain of the agreements require the Company to pay minimum royalties or license maintenance fees. The Company recognized $69,854 and $23,038 of royalty expense for the three months ended March 31, 2016 and 2015, respectively. In 2016, future minimum royalty fees are approximately $270,000 under these agreements.

In September 2013, the Company entered into a technology development agreement in which the Company would receive fixed milestone payments for meeting development milestones under the agreement. Since the milestones are substantive, the Company recognizes revenue in the periods in which the substantive milestones are achieved. In addition, the Company received an upfront payment of $250,000, which is recognized on a straight-line basis over the term of the technology development agreement which ended in December 2015. The Company recognized total revenue of $252,780 during the three months ended March 31, 2015 (none in 2016) relating to this arrangement.

Related party transactions

3 Months Ended

Mar. 31, 2016

Related party transactions

Note 11 - Related party transactions

In March 2014, the Company entered into a supply agreement with Fluidigm Corporation (“Fluidigm”) under which Fluidigm supplies the Company with its microfluidic test platform for use in manufacturing the Acuitas MDRO Gene Test. The Company’s CEO and Chairman of the Board of Directors is a director of Fluidigm. On July 12, 2015, the Company entered into a letter agreement (the “Fluidigm Agreement”) with Fluidigm to expand the companies’ existing relationship to include collaborating on the development of test kits and custom analytic instruments for identification, screening and surveillance testing of MDROs. The Fluidigm Agreement also expands the existing Supply Agreement between the Company and Fluidigm, and provides for expansion of the gene targets and organisms to be tested on the Company’s existing CLIA lab-based tests, the Acuitas MDRO Gene Test and the Acuitas Resistome Test, using Fluidigm technologies and products. Additionally, Fluidigm has agreed not to develop or directly collaborate with any third party to develop an FDA approved or CE-marked diagnostic test for the purpose of detecting resistome genes for identified MDROs if the Company meets certain minimum purchase commitments and other requirements. The initial term of the Fluidigm Agreement is five years. Both parties have the ability to extend the term for an additional five years. Under the expanded Supply Agreement, the term is extended until March 17, 2018, and the Company has the right to extend the term of the Supply Agreement for up to two additional three-year terms. The Company paid $93,224 and $87,028 related to these agreements in the three months ended March 31, 2016 and 2015, respectively.

In the three months ended March 31, 2016 and 2015, The Company incurred $67,775 and $83,516, respectively, of inventory purchases under the agreements with Fluidigm. In addition, the Company has several capital lease arrangements for laboratory equipment manufactured by Fluidigm. The Company paid $45,106 and $14,853 related to the leased equipment in the 2016 and 2015 quarterly periods, respectively.

- Definition

The entire disclosure for related party transactions. Examples of related party transactions include transactions between (a) a parent company and its subsidiary; (b) subsidiaries of a common parent; (c) and entity and its principal owners; and (d) affiliates.

+ References + Details

Subsequent events

3 Months Ended

Mar. 31, 2016

Subsequent Events [Abstract]

Subsequent events

Note 12 – Subsequent events

On April 28, 2016, the Board of Directors made a stock option award to the Company’s Chief Executive Officer and Chair of the Board. The non-qualified stock option award to acquire 766,500 shares of common stock represents approximately 6% of outstanding shares of common stock as of the record date for the Company’s upcoming 2016 Annual Meeting of Stockholders. The stock option grant has an exercise price of $1.35 per share, which is the fair market value of the common stock on the date of grant, a ten year term and a vesting schedule of 25% vesting of the award on the first annual anniversary of the date of grant and then 6.25% vesting each quarter thereafter over three additional years. The stock option award is contingent on receipt of stockholder approval at the Company’s 2016 Annual Meeting of Stockholders, as the award was made outside of the Company’s stockholder-approved incentive plans. If the stockholders do not approve the award, the stock option award will terminate.

Summary of significant accounting policies (Policies)

3 Months Ended

Mar. 31, 2016

Accounting Policies [Abstract]

Basis of Presentation

Basis of presentation and consolidation

Foreign Currency

Unless otherwise noted, all references to “$” or “dollar” refer to the U.S. dollar.

Use of Estimates

Use of estimates

Fair value of financial instruments

For additional fair value disclosures, see Note 6.

Cash and cash equivalents and restricted cash

Cash and cash equivalents

Accounts Receivable

Accounts receivable

Inventory

Inventories

Inventories are valued using the first-in, first-out method and stated at the lower of cost or market and consist of the following:

	March 31, 2016		December 31, 2015

Raw materials and supplies	$	600,044	$	362,526
Work-in process		127,130		150,369
Finished goods		197,171		313,117
Total	$	924,345	$	826,012

Property and equipment

Intangible assets and goodwill

Intangible assets and goodwill as of March 31, 2016 were acquired as part of the Merger, and consist of definite-lived intangible assets and goodwill.

Definite-lived intangible assets

	Cost		Accumulated Amortization		Net balance at March 31, 2016

Trademarks and tradenames	$	461,000	$	(32,838)	$	428,162
Developed technology		458,000		(46,607)		411,393
Customer relationships		1,094,000		(111,695)		982,305
	$	2,013,000	$	(191,140)	$	1,821,860

Goodwill

Redeemable convertible preferred stock

Revenue recognition

Amounts billed to customers for shipping and handling are included in revenue when the related product or service revenue is recognized. Shipping and handling costs are included in cost of sales.

Revenue from sales of the Argus System

Revenue from sales of QuickFISH, PNA FISH and XpressFISH diagnostic test products

Revenue is recognized upon shipment to the customer. Sales are recorded net of accruals for estimated rebates, discounts and other deductions and returns.

Revenue from sales of Genome Builder Software and consumables (on a stand-alone basis)

Revenue is recognized for Genome Builder Software and for consumables, when sold on a standalone basis, upon delivery to the customer.

Revenue from extended warranty service contracts

The Company recognizes revenue associated with extended warranty service contracts over the service period in proportion to the costs expected to be incurred over that same period.

Revenue from providing laboratory services

The Company recognizes revenue associated with laboratory services contracts when the service has been performed and reports are made available to the customer.

Revenue from funded software development arrangements

Share-based compensation

Income taxes

Loss per share

Basic loss per share is computed by dividing net loss available to common stockholders by the weighted average number of shares of common stock outstanding during the period.

Recent accounting pronouncements

Summary of significant accounting policies (Tables)

3 Months Ended

Mar. 31, 2016

Accounting Policies [Abstract]

Schedule of inventories

Inventories are valued using the first-in, first-out method and stated at the lower of cost or market and consist of the following:

	March 31, 2016		December 31, 2015

Raw materials and supplies	$	600,044	$	362,526
Work-in process		127,130		150,369
Finished goods		197,171		313,117
Total	$	924,345	$	826,012

Schedule of Finite-Lived Intangible Assets

	Cost		Accumulated Amortization		Net balance at March 31, 2016

Trademarks and tradenames	$	461,000	$	(32,838)	$	428,162
Developed technology		458,000		(46,607)		411,393
Customer relationships		1,094,000		(111,695)		982,305
	$	2,013,000	$	(191,140)	$	1,821,860

Business Combination (Tables)

3 Months Ended

Mar. 31, 2016

Business Combinations [Abstract]

Schedule of preliminary allocation of the purchase price

The following represents the preliminary allocation of the purchase price (as adjusted for measurement period adjustments):

Total purchase price - fair value of common stock issued	$	2,584,090

Fair value of tangible assets acquired:
Cash	$	1,367,211
Receivables		536,406
Inventory		881,273
Property and equipment		245,479
Other assets		359,587
Fair value of identifable intangible assets acquired:
Customer relationships		1,094,000
Developed technology		458,000
Trademarks and tradenames		461,000
Fair value of goodwill		600,814

Deferred tax liabilities, net		129,095
Fair value of liabilities assumed		3,290,585
	$	2,584,090

Schedule of Change in Accounting Estimate

the Company identified an additional adjustment to the provisional estimated fair values, as follows:

	As previously reported (1)		Adjustment		Adjusted amount

Accounts payable	$	2,285,792	$	(36,714)	$	2,249,078
Goodwill		637,528		(36,714)		600,814

(1) As reported on Form 10-K for the year ended December 31, 2015

Schedule of unaudited pro forma financial information

The pro forma amounts do not purport to be indicative of the results that would have actually been obtained if the acquisition occurred as of January 1, 2015 or that may be obtained in the future:

	Three Months Ended March 31,
Unaudited pro forma results	2015

Revenues	$	1,429,686
Net loss	$	(3,797,029)
Net loss per share	$	(3.38)

Fair value measurements (Tables)

3 Months Ended

Mar. 31, 2016

Fair Value Disclosures [Abstract]

Schedule of financial assets and liabilities measured at fair value on a recurring basis

The following tables set forth a summary of changes in the fair value of Level 3 liabilities measured at fair value on a recurring basis for the year ended December 31, 2015:

	Balance at		Established		Change in		Reclassified		Balance at
Description	December 31, 2014		in 2015		Fair Value		to Equity		December 31, 2015

Derivative warrant liability	$	-	$	72,333	$	647,342	$	(719,675)	$	-

Stockholders' equity (Tables)

3 Months Ended

Mar. 31, 2016

Stockholders' Equity Note [Abstract]

Schedule of allocation of share-based compensation expense

The allocation of share-based compensation expense by operating expenses is as follows:

	Three months ended March 31,
	2016		2015
Costs of services	$	4,312	$	-
Research and development		62,218		36,456
General and administrative		172,103		83,299
Sales and marketing		22,864		470,848
	$	261,497	$	590,603

Schedule of warrants to purchase shares of common stock

the following warrants to purchase shares of common stock were outstanding:

Issuance	Exercise Price		Expiration	Shares of Common Stock Subject to Warrants
August 2007	$	7.91	August 2017		8,921
March 2008	$	790.54	March 2018		46
November 2009	$	7.91	November 2019		6,674
January 2010	$	7.91	January 2020		6,674
March 2010	$	7.91	March 2020		1,277
November 2011	$	7.91	November 2021		5,213
December 2011	$	7.91	December 2021		664
March 2012	$	109.90	March 2019		4,125
February 2015	$	6.60	February 2025		225,011
May 2015	$	6.60	May 2020		3,457,750
					3,716,355

Liquidity and management's plans (Details Textual) - USD ($)			1 Months Ended		3 Months Ended			6 Months Ended	12 Months Ended
	Jul. 14, 2015	May 08, 2015	May 31, 2016	May 31, 2015	Mar. 31, 2016	Jun. 30, 2015	Mar. 31, 2015	Mar. 31, 2016	Dec. 31, 2015
Conversion of Stock [Line Items]
Proceeds from Notes Payable					$ 0		$ 500,000
Units Of Stock And Warrants Issued During Period Shares Initial Public Offering		2,850,000
Stock And Warrants Issued During Period Shares Initial Public Offering Purchase Price Per Unit		$ 6.00
Proceeds From Issuance Of Units Under Initial Public Offering Net		$ 12,100,000
Proceeds from Issuance Initial Public Offering		$ 17,100,000			$ 0		(252,715)
Common Stock
Conversion of Stock [Line Items]
Warrants Issued During Period On Exercise Of Over Allotment Option Shares		422,500
Shares Issued, Price Per Share		$ 6.00
Common Stock \| Merck Global Health Innovation Fund LLC
Conversion of Stock [Line Items]
Proceeds From Issuance Of Shares And Debt	$ 6,000,000
Stock Issued During Period, Shares, New Issues	1,136,364
Shares Issued, Price Per Share	$ 4.40
Initial public offering
Conversion of Stock [Line Items]
Conversion of Stock, Shares Issued		7,374,852
Debt Conversion, Converted Instrument, Amount		$ 2,100,000
Debt Conversion, Converted Instrument, Shares Issued		350,000		1,875,000
Proceeds from Issuance Initial Public Offering		$ 12,100,000
Initial public offering \| Common Stock
Conversion of Stock [Line Items]
Debt Conversion, Converted Instrument, Shares Issued				350,000
Convertible Notes Payable
Conversion of Stock [Line Items]
Proceeds from Convertible Debt									$ 1,500,000
Short-term Demand Notes
Conversion of Stock [Line Items]
Proceeds from Notes Payable								$ 2,300,000	$ 800,000
Repayments of Notes Payable						$ 200,000
Debt Conversion, Converted Instrument, Amount							$ 300,000
Promissory Note \| Common Stock \| Merck Global Health Innovation Fund LLC
Conversion of Stock [Line Items]
Debt Instrument, Face Amount	$ 1,000,000
Proceeds From Issuance Of Common Stock And Notes Payable	$ 6,000,000
Subsequent Event
Conversion of Stock [Line Items]
Proceeds from Issuance of Private Placement			$ 10,400,000
Class of Warrant or Right, Exercise Price of Warrants or Rights			$ 0.75

Summary of significant accounting policies (Details) - USD ($)	Mar. 31, 2016	Dec. 31, 2015
Inventories
Raw materials and supplies	$ 600,044	$ 362,526
Work-in process	127,130	150,369
Finished goods	197,171	313,117
Total	$ 924,345	$ 826,012

Summary of significant accounting policies (Details 1) - USD ($)	Mar. 31, 2016	Dec. 31, 2015
Finite-Lived Intangible Assets [Line Items]
Cost	$ 2,013,000
Accumulated Amortization	(191,140)
Net balance at March 31, 2016	1,821,860	$ 1,888,814
Trademarks and tradenames
Finite-Lived Intangible Assets [Line Items]
Cost	461,000
Accumulated Amortization	(32,838)
Net balance at March 31, 2016	428,162
Developed technology
Finite-Lived Intangible Assets [Line Items]
Cost	458,000
Accumulated Amortization	(46,607)
Net balance at March 31, 2016	411,393
Customer relationships
Finite-Lived Intangible Assets [Line Items]
Cost	1,094,000
Accumulated Amortization	(111,695)
Net balance at March 31, 2016	$ 982,305

Summary of significant accounting policies (Details Textual) - USD ($)	3 Months Ended
	Mar. 31, 2016	Mar. 31, 2015	Dec. 31, 2015	May 08, 2015
Significant Accounting Policies [Line Items]
Allowance for Doubtful Accounts Receivable	$ 13,352		$ 15,596
Inventory Valuation Reserves	$ 614,109		599,064
Operating Loss Carryforwards			90,297,000
Antidilutive Securities Excluded from Computation of Earnings Per Share, Amount	5,900,000	8,900,000
FDIC limit of insurable cash	$ 250,000
Goodwill	600,814		637,528
Letters of Credit Outstanding, Amount	243,380		$ 243,380
Amortization of Intangible Assets	66,954
Finite-Lived Intangible Assets, Amortization Expense, Next Twelve Months	$ 268,000
Developed Technology Rights [Member]
Significant Accounting Policies [Line Items]
Finite-Lived Intangible Asset, Useful Life	7 years
Customer Relationships [Member]
Significant Accounting Policies [Line Items]
Finite-Lived Intangible Asset, Useful Life	7 years
Trademarks [Member]
Significant Accounting Policies [Line Items]
Finite-Lived Intangible Asset, Useful Life	10 years
Common Stock [Member]
Significant Accounting Policies [Line Items]
Convertible Preferred Stock, Shares Issued upon Conversion				7,374,852
Series A Convertible Redeemable Preferred Stock
Significant Accounting Policies [Line Items]
Temporary Equity Redemption Request Of Minimum Percentage Of Holders Of Outstanding Preferred Stock Required	70.00%
Temporary Equity, Shares Outstanding		3,999,864
Accounts Receivable \| Customer One
Significant Accounting Policies [Line Items]
Concentration Risk, Percentage	10.00%
Sales Revenue, Net [Member] \| Hitachi High - Technologies Corporation
Significant Accounting Policies [Line Items]
Concentration Risk, Percentage	10.00%	54.00%
Maximum
Significant Accounting Policies [Line Items]
Accounts Receivable Period Due	60 days
Minimum
Significant Accounting Policies [Line Items]
Accounts Receivable Period Due	30 days

Business Combination (Details) - USD ($)	Mar. 31, 2016	Dec. 31, 2015
Fair value of identifable intangible assets acquired:
Fair value of goodwill	$ 600,814	$ 637,528
AdvanDx [Member]
Business Acquisition [Line Items]
Total purchase price - fair value of common stock issued	2,584,090
Fair value of tangible assets acquired:
Cash	1,367,211
Receivables	536,406
Inventory	881,273
Property and equipment	245,479
Other assets	359,587
Fair value of identifable intangible assets acquired:
Customer relationships	1,094,000
Developed technology	458,000
Trademarks and tradenames	461,000
Fair value of goodwill	600,814
Deferred tax liabilities, net	129,095
Fair value of liabilities assumed	3,290,585
Total acquisition purchase price Allocation	$ 2,584,090

Business Combination (Details 1) - USD ($)

Mar. 31, 2016

Dec. 31, 2015

Business Acquisition [Line Items]

Accounts payable

$ 2,205,538

$ 2,285,792

Goodwill

600,814

$ 637,528

Scenario, Previously Reported [Member]

Business Acquisition [Line Items]

Accounts payable

^[1]

2,285,792

Goodwill

^[1]

637,528

Scenario, Adjustment [Member]

Business Acquisition [Line Items]

Accounts payable

(36,714)

Goodwill

$ (36,714)

[1]	As reported on Form 10-K for the year ended December 31, 2015

Business Combination (Details 2)	3 Months Ended
Business Combination (Details 2)	Mar. 31, 2015 USD ($) $ / shares
Business Acquisition, Pro Forma Information [Abstract]
Revenues	$ 1,429,686
Net loss	$ (3,797,029)
Net loss per share \| $ / shares	$ (3.38)

Business Combination (Details Textual) - USD ($)		12 Months Ended
Business Combination (Details Textual) - USD ($)	Jul. 14, 2015	Dec. 31, 2015	Mar. 31, 2016
Business Acquisition [Line Items]
Goodwill		$ 637,528	$ 600,814
Payments of Merger Related Costs, Financing Activities	$ 500,000
Deferred Tax Assets, Tax Credit Carryforwards, Foreign	4,300,000
Deferred Tax Liabilities, Net, Total	$ 100,000
Goodwill, Period Increase (Decrease), Total		$ 345,781
AdvanDx [Member]
Business Acquisition [Line Items]
Goodwill			$ 600,814
AdvanDx [Member] \| Equity Issued in Business Combination [Member]
Business Acquisition [Line Items]
Business Acquisition, Equity Interest Issued or Issuable, Number of Shares	681,818
Equity Issued in Business Combination, Fair Value Disclosure	$ 2,600,000

Merck GHI Financing (Details Textual) - Merck GHI Financing Agreement [Member] - USD ($)	Jul. 14, 2015	Dec. 31, 2015	Jul. 31, 2015
Common Stock and Note Purchase Agreement [Line Items]
Stock Issued During Period, Shares, New Issues	1,136,364
Shares Issued, Price Per Share	$ 4.40
Proceeds from Issuance of Common Stock	$ 5,000,000
Debt Instrument, Interest Rate, Stated Percentage	8.00%		8.00%
Debt Instrument, Face Amount	$ 1,000,000	$ 1,000,000
Fees Related to Common Stock and Debt Transaction	50,000
Payments of Debt Issuance Costs	8,000
Adjustments to Additional Paid in Capital, Stock Issued, Issuance Costs	$ 42,000

Fair value measurements (Details) - Warrant	12 Months Ended
Fair value measurements (Details) - Warrant	Dec. 31, 2015 USD ($)
Changes in the fair value of Level 3 liabilities measured at fair value on recurring basis
Balance at the begining of the period	$ 0
Established in 2015	72,333
Change in Fair Value	647,342
Reclassified to Equity	(719,675)
Balance at the end of the period	$ 0

Debt (Details Textual)		1 Months Ended				2 Months Ended	3 Months Ended			6 Months Ended	12 Months Ended
Debt (Details Textual)	May 08, 2015 USD ($) shares	Jul. 31, 2015 USD ($)	May 31, 2015 USD ($) shares	Apr. 30, 2015 USD ($)	Jun. 30, 2013 USD ($)	Mar. 31, 2015 USD ($) $ / shares	Mar. 31, 2016 USD ($)	Mar. 31, 2015 USD ($) $ / shares	Sep. 30, 2014 USD ($)	Mar. 31, 2016 USD ($)	Dec. 31, 2015 USD ($) shares	Jul. 14, 2015 USD ($)	Dec. 31, 2009 USD ($)
Debt Instrument [Line Items]
Proceeds from Notes Payable							$ 0	$ 500,000
Interest Expense, Debt			$ 1,500,000				31,934	96,397
Amortization of Financing Costs			$ 71,421
Repayments of Debt				$ 150,000			$ 0	$ 1,250
Merck GHI Financing Agreement [Member]
Debt Instrument [Line Items]
Debt Instrument, Interest Rate, Stated Percentage		8.00%										8.00%
Proceeds from Other Short-term Debt		$ 1,000,000
Debt Instrument, Face Amount											$ 1,000,000	$ 1,000,000
Department of Business and Economic Development Agreement [Member]
Debt Instrument [Line Items]
Debt Instrument, Face Amount					$ 100,000
Repayments of Debt					75,000
Interest Payable					$ 11,811
Loan From Department Of Business And Economic Development, Maryland [Member]
Debt Instrument [Line Items]
Debt Instrument, Interest Rate, Stated Percentage													3.00%
Debt Instrument, Face Amount													$ 100,000
Loan From Department Of Business And Economic Development, Montgomery County [Member]
Debt Instrument [Line Items]
Debt Instrument, Face Amount													$ 10,000
IPO [Member]
Debt Instrument [Line Items]
Debt Conversion, Converted Instrument, Amount	$ 2,100,000
Debt Conversion, Converted Instrument, Shares Issued \| shares	350,000		1,875,000
Interest Expense, Debt			$ 1,500,000
Common Stock Percent											110.00%
IPO [Member] \| Common Stock [Member]
Debt Instrument [Line Items]
Debt Conversion, Converted Instrument, Shares Issued \| shares			350,000
2014 Convertible Notes
Debt Instrument [Line Items]
Debt Instrument, Interest Rate, Stated Percentage									8.00%
Proceeds from Convertible Debt									$ 1,500,000
2015 Convertible Notes
Debt Instrument [Line Items]
Debt Instrument, Interest Rate, Stated Percentage						8.00%		8.00%
Proceeds from Other Short-term Debt						$ 1,500,000
Fair Value Adjustment of Warrants	$ 700,000
2015 Convertible Notes \| Common Stock [Member]
Debt Instrument [Line Items]
Class of Warrant or Right, Number of Securities Called by Warrants or Rights \| shares											225,011
Debt Instrument, Convertible, Conversion Price \| $ / shares						$ 1.00		$ 1.00
2015 Convertible Notes \| Pre IPO
Debt Instrument [Line Items]
Debt Instrument, Convertible, Conversion Ratio						1.25
2015 Convertible Notes \| Post IPO
Debt Instrument [Line Items]
Debt Instrument, Convertible, Conversion Ratio						1.00
Short-term Demand Notes
Debt Instrument [Line Items]
Proceeds from Notes Payable										$ 2,300,000	$ 800,000
Debt Conversion, Converted Instrument, Amount								$ 300,000
Debt Instrument, Interest Rate, Stated Percentage			8.00%

Stockholders' equity (Details) - USD ($)	3 Months Ended
Stockholders' equity (Details) - USD ($)	Mar. 31, 2016	Mar. 31, 2015
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Allocated share-based compensation expense	$ 261,497	$ 590,603
Costs of services [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Allocated share-based compensation expense	4,312	0
Research and development
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Allocated share-based compensation expense	62,218	36,456
General and administrative
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Allocated share-based compensation expense	172,103	83,299
Sales and marketing
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Allocated share-based compensation expense	$ 22,864	$ 470,848

Stockholders' equity (Details 1)	3 Months Ended
Stockholders' equity (Details 1)	Mar. 31, 2016 $ / shares shares
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Shares of Common Stock Subject to Warrants	3,716,355
August 2007
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 7.91
Expiration	2017-08
Shares of Common Stock Subject to Warrants	8,921
March 2008
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 790.54
Expiration	2018-03
Shares of Common Stock Subject to Warrants	46
November 2009
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 7.91
Expiration	2019-11
Shares of Common Stock Subject to Warrants	6,674
January 2010
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 7.91
Expiration	2020-01
Shares of Common Stock Subject to Warrants	6,674
March 2010
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 7.91
Expiration	2020-03
Shares of Common Stock Subject to Warrants	1,277
November 2011
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 7.91
Expiration	2021-11
Shares of Common Stock Subject to Warrants	5,213
December 2011
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 7.91
Expiration	2021-12
Shares of Common Stock Subject to Warrants	664
March 2012
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 109.90
Expiration	2019-03
Shares of Common Stock Subject to Warrants	4,125
February 2015
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 6.60
Expiration	2025-02
Shares of Common Stock Subject to Warrants	225,011
May 2015
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Exercise Price \| $ / shares	$ 6.60
Expiration	2020-05
Shares of Common Stock Subject to Warrants	3,457,750

Stockholders' equity (Details Textual) - USD ($)			1 Months Ended			3 Months Ended
Stockholders' equity (Details Textual) - USD ($)	Jul. 14, 2015	May 08, 2015	May 31, 2015	Oct. 24, 2014	Mar. 31, 2014	Mar. 31, 2016	Dec. 31, 2015	Mar. 31, 2015
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Allocated Share-based Compensation Expense						$ 261,497		$ 590,603
Share-based Compensation Arrangement by Share-based Payment Award, Options, Grants in Period, Gross						30,000
Share-based Compensation Arrangements by Share-based Payment Award, Options, Grants in Period, Weighted Average Exercise Price						$ 1.53
Proceeds From Issuance Of Units Under Initial Public Offering Gross		$ 17,100,000
Proceeds From Issuance Of Units Under Initial Public Offering Net		$ 12,100,000
Common Stock, Shares Authorized						200,000,000	200,000,000
Common Stock, Shares, Issued						12,576,306	12,547,644
Preferred Stock, Shares Authorized						10,000,000	10,000,000	10,000,000
Over-Allotment Option [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Class of Warrant or Right, Number of Securities Called by Warrants or Rights		3,272,500
Class of Warrant or Right, Exercise Price of Warrants or Rights		$ 6.60
Expiration Date of Warrants Issued		May 08, 2020
IPO [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Debt Conversion, Converted Instrument, Amount		$ 2,100,000
Debt Conversion, Converted Instrument, Shares Issued		350,000	1,875,000
Demand Notes Payable [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Debt Conversion, Converted Instrument, Amount		$ 2,100,000
2015 Plan
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Share-based Compensation Arrangement by Share-based Payment Award, Number of Shares Authorized						1,355,000
Share-based Compensation Arrangement by Share-based Payment Award, Number of Shares Available for Grant						748,479
Share-based Compensation Arrangement by Share-based Payment Award, Common stock Percentage						4.00%
2015 Plan \| January 1, 2016 [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Share-based Compensation Arrangement by Share-based Payment Award, Number of Shares Available for Grant						501,907
Stock options
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Share-based Compensation Arrangement by Share-based Payment Award, Options, Grants in Period, Weighted Average Grant Date Fair Value						$ 0.73
Share-based Compensation Arrangement by Share-based Payment Award, Options, Outstanding, Number						2,119,140
Restricted stock units
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Share-based Compensation Arrangement by Share-based Payment Award, Equity Instruments Other than Options, Grants in Period					130,640		75,000
Share-based Compensation Arrangement by Share-based Payment Award, Equity Instruments Other than Options, Grants in Period, Weighted Average Grant Date Fair Value							$ 1.70
Common Stock [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Stock and Warrants Issued in Conjunction with Initial Public Offering		2,850,000
Offering Price Per Unit		$ 6.00
Adjustments to Additional Paid in Capital, Stock Issued, Issuance Costs		$ 2,900,000
Warrants Issued During Period On Exercise Of Over Allotment Option Shares		422,500
Proceeds from Issuance of Warrants		$ 4,225
Convertible Preferred Stock, Shares Issued upon Conversion		7,374,852
Shares Of Stock Issued For Restricted Stock Unit Exercises				130,640
Common Stock [Member] \| Merck Global Health Innovation Fund LLC [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Stock Issued During Period, Shares, New Issues	1,136,364
Offering Price Per Unit	$ 4.40
Proceeds from Issuance of Common Stock	$ 5,000,000
Common Stock [Member] \| Over-Allotment Option [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Class of Warrant or Right, Number of Securities Called by Warrants or Rights		185,250
Common Stock [Member] \| IPO [Member]
Share-based Compensation Arrangement by Share-based Payment Award [Line Items]
Debt Conversion, Converted Instrument, Shares Issued			350,000

Commitments and contingencies (Details Textual) - USD ($)	3 Months Ended
	Mar. 31, 2016	Mar. 31, 2015
Loss Contingencies [Line Items]
Operating Leases, Rent Expense, Net, Total	$ 254,044	$ 183,154

License agreements, research collaborations and development agreements (Details Textual)	1 Months Ended	3 Months Ended
	Sep. 30, 2013 USD ($)	Mar. 31, 2016 USD ($)	Mar. 31, 2015 USD ($)
License Agreements Research Collaborations And Development Agreements [Line Items]
Future Minimum Royalty Payments Due		$ 270,000
License Upfront Payment Received	$ 250,000
Royalty (Income) Expense		69,854	$ 23,038
Licenses Revenue		$ 0	$ 252,780
Number Of License Agreements		3

Related party transactions (Details Textual) - USD ($)	3 Months Ended
Related party transactions (Details Textual) - USD ($)	Mar. 31, 2016	Mar. 31, 2015
Related Party Transaction [Line Items]
Related Party Transaction, Purchases from Related Party	$ 67,775	$ 83,516
Related Party Transaction, Expenses from Transactions with Related Party	93,224	87,028
Payments to Acquire Equipment on Lease	$ 45,106	$ 14,853

Subsequent Events (Details Textual) - USD ($) $ / shares in Units, $ in Millions	1 Months Ended		3 Months Ended
	May 31, 2016	Apr. 30, 2016	Mar. 31, 2016
Subsequent Event [Line Items]
Share-based Compensation Arrangement by Share-based Payment Award, Options, Grants in Period, Gross			30,000
Subsequent Event
Subsequent Event [Line Items]
Proceeds from Issuance of Private Placement	$ 10.4
Subsequent Event \| Chief Executive Officer [Member]
Subsequent Event [Line Items]
Share-based Compensation Arrangement by Share-based Payment Award, Award Vesting Rights		vesting schedule of 25% vesting of the award on the first annual anniversary of the date of grant and then 6.25% vesting each quarter thereafter over three additional years
Share-based Compensation Arrangement by Share-based Payment Award, Options, Grants in Period, Gross		766,500
Percentage of Total Outstanding Shares of Common Stock		6.00%
Share Based Compensation Arrangements By Share Based Payment Award contractual Term		10 years
Share Based Compensation Arrangements By Share Based Payment Award Options Grants In Period Exercise Price		$ 1.35